NEUROD1 antibody

Principal name

NEUROD1 antibody

Alternative names for NEUROD1 antibody

NEUROD1, NEUROD, Neurogenic differentiation factor 1

SwissProt ID

P79765 (Chick), Q13562 (Human), Q60867 (Mouse), Q64289 (Rat)

Gene ID

4760 (NEUROD1), 18012 (Neurod1), 29458

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog, Eq (Equine), Por (Porcine), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C)

Background of NEUROD1 antibody

The NEUROD1 gene encodes a member of the NeuroD family of basic-loop-helix transcription factors.  The NEUROD 1 was first described by Lee et al (1995) who observed that the gene was transiently expressed in subsets of neuronal cells of the central and peripheral nervous system at the time of their terminal differentiation into mature neurons. They also showed that ectopic expression of the NEUROD1 gene could convert non-neuronal cells to neuronal cells. The NEUROD1 protein forms heterodimers with other bHLH proteins and activates the transcription of a large set of genes that contain the E-box DNA sequence 5’-CANNTG’-3.  NEUROD1 is involved in the regulation of several cell differentiation pathways including the development of retinal ganglion cell, inner ear sensory neurons, and endocrine islet cells. NeuroD1 regulates the expression of numerous genes including the insulin gene. Mutations in the NEUROD1 gene has been associated with type II diabetes mellitus. Defects in expression of  NEUROD1 is linked with Maturity onset diabetes of the young type 6 (MODY6).  The NEUROD1 protein may be localized in the cytoplasm and the nucleus.  In Min 6 insulinoma cells Petersen et al (2002) were able to conclude that MEK-ERK associated phosphorylation of the serine 274 on NEUROD1 induces the translocation of the protein to the nucleus and that this may normally be associated with cell glucose concentration.

General readings

Peng,S.Y., et al., (2005) Biochim. Biophys. Acta 1731 (3), 154-159

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Anticorps Primaires

N° du Produit Hôte Isotype Clone Presentation Reactivité Applications  

NEUROD1 (N-term) anticorps

  Rabbit polyclonal NeuroD1 Antibody (N-term)  
Western blot analysis of hNeuroD1-Q30 (Cat. #TA324696) in HepG2 cell line lysates (35ug/lane). NEUROD1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu P
0.4 ml / 410,00 €
  OriGene Technologies, Inc.

NEUROD1 anticorps

  Rabbit polyclonal Neuro D (Ab-274) antibody  
Western blot analysis of extracts from HeLa cells, using Neuro D (Ab-274) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / 335,00 €
  OriGene Technologies, Inc.

NEUROD1 anticorps

  Rabbit polyclonal Neuro D (Ser274) antibody(Phospho-specific)  
Western blot analysis of extracts from HeLa cells, treated with UV (15mins), using Neuro D (Phospho-Ser274) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / 335,00 €
  OriGene Technologies, Inc.

NEUROD1 (C-term) anticorps

  Rabbit polyclonal NeuroD1 Antibody (C-term)  
NeuroD1 Antibody (I333) (Cat. #TA324697) western blot analysis in Y79 cell line ,mouse cerebellum and rat brain tissue lysates (35ug/lane).This demonstrates the NeuroD1 antibody detected the NeuroD1 protein (arrow). Rabbit Ig Purified Hu, Ms WB
0.4 ml / 410,00 €
  OriGene Technologies, Inc.

NEUROD1 anticorps

  Rabbit Polyclonal Anti-NEUROD1 Antibody  
WB Suggested Anti-NEUROD1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / 360,00 €
  OriGene Technologies, Inc.

NEUROD1 anticorps

  Rabbit Polyclonal Anti-NEUROD1 Antibody  
WB Suggested Anti-NEUROD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Eq, Hu, Ms, Por, Rt, Sh WB
50 µg / 360,00 €
  OriGene Technologies, Inc.

NEUROD1 anticorps

  Rabbit Polyclonal Anti-Neuro D Antibody  
Western blot analysis on HeLa cell lysate using Neuro D Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / 335,00 €
  OriGene Technologies, Inc.

NEUROD1 (201-300) anticorps

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG 3D11 Purified Hu E, P, WB
50 µg / 420,00 €
  OriGene Technologies GmbH

NEUROD1 anticorps

Figure 1. Shows Neuro2A (Mouse neuroblastoma) and SKNSH, 1691 and IMR32 (Human neuroblastoma) cells stained with anti-NEUROD1 Antibody Cat.-No AM32710PU-N (Clone BC18-1.11EG) by the Indirect Immunofluorescence method. The secondary antibody was FITC conjugated anti-Mouse Ig. Nuclear and cytoplasmic staining for NEUROD is observed in all cells. Since the cell lines tested loosely attach to surfaces of chamber slides, the cells were immobilized on adhesion slides for the staining proceedure. The cells were allowed to attach to adhesion slides, fixed in 1% paraformaldehyde, permeabilized in 0.25% Triton X 100 in PBS, blocked with 1% BSA and stained overnight with 5 µg/ml antibody diluted with 1% BSA in PBS. The secondary antibody was diluted with 1% BSA in PBS according to manufacturers instruction and incubated for 2 hours. Mouse IgG1 BC18-1.11EG Purified Hu, Ms C, ICC/IF, WB
0.1 mg / 250,00 €
  OriGene Technologies GmbH

NEUROD1 pSer274 anticorps

Western blot analysis of extracts from HeLa cells treated with UV using Neuro D (Phospho-Ser274) Antibody AP55826PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / 340,00 €
  OriGene Technologies GmbH

NEUROD1 pSer274 anticorps

Western blot analysis of extracts from HeLa cells treated with UV using Neuro D (Phospho-Ser274) Antibody AP55826PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / 270,00 €
  OriGene Technologies GmbH

NEUROD1 pSer274 anticorps

Western blot analysis of extracts from HeLa cells treated with UV 15', using Neuro D (Phospho-Ser274) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / 230,00 €
  Assay Biotech

NEUROD1 pSer274 anticorps

Western blot analysis of extracts from HeLa cells treated with UV 15', using Neuro D (Phospho-Ser274) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / 330,00 €
  Assay Biotech

NEUROD1 anticorps

NeuroD1 staining of immersion fixed beta TC-6 mouse beta cell insulinoma cell line  at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Fluoro-labeled ™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Protocol on Datasheet. Goat IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 mg / 450,00 €
  Neuromics Antibodies

Proteins & Growth Factors

N° du Produit Espèces Presentation Pureté Source  

NEUROD1

  Recombinant protein of human neurogenic differentiation 1 (NEUROD1)  
NEUROD1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 438,00 €
  OriGene Technologies, Inc.

Lysates

N° du Produit    

NEUROD1 overexpression lysate

NEUROD1 overexpression lysate  
0.1 mg / 295,00 €
  OriGene Technologies, Inc.

Human Lenti ORF Particles

N° du Produit    

Lenti ORF particles, NEUROD1 (mGFP-tagged) - Human neurogenic differentiation 1 (NEUROD1), 200 uL, >10^7 TU/mL

Lenti ORF particles, NEUROD1 (mGFP-tagged) - Human neurogenic differentiation 1 (NEUROD1), 200 uL, >10^7 TU/mL  
200 µl / 1 082,00 €
  OriGene Technologies, Inc.

Lenti ORF particles, NEUROD1 (Myc-DDK tagged) - Human neurogenic differentiation 1 (NEUROD1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, NEUROD1 (Myc-DDK tagged) - Human neurogenic differentiation 1 (NEUROD1) , 200 uL, >10^7 TU/mL  
200 µl / 969,00 €
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

N° du Produit    

Lenti ORF particles, Neurod1 (GFP-tagged) - Mouse neurogenic differentiation 1 (Neurod1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Neurod1 (GFP-tagged) - Mouse neurogenic differentiation 1 (Neurod1), 200 uL, >10^7 TU/mL  
200 µl / 907,00 €
  OriGene Technologies, Inc.

Lenti ORF particles, Neurod1 (Myc-DDK-tagged) - Mouse neurogenic differentiation 1 (Neurod1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Neurod1 (Myc-DDK-tagged) - Mouse neurogenic differentiation 1 (Neurod1), 200 uL, >10^7 TU/mL  
200 µl / 804,00 €
  OriGene Technologies, Inc.

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