DNA ligase 1 antibody

Principal name

DNA ligase 1 antibody

Alternative names for DNA ligase 1 antibody

LIG1, LIG-1, DNA ligase 1, EC=6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1

SwissProt ID

P18858 (Human), P37913 (Mouse), Q9JHY8 (Rat)

Gene ID

3978 (LIG1), 16881 (Lig1), 81513

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, GP (Guinea Pig), Rb (Rabbit), Eq (Equine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of DNA ligase 1 antibody

Kit Component:
- KN309280G1, Lig1 gRNA vector 1 in pCas-Guide vector
- KN309280G2, Lig1 gRNA vector 2 in pCas-Guide vector
- KN309280D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Arora, M., et al. Leukemia 24(8):1470-1475(2010)
Guillem, V.M., et al. Am. J. Hematol. 85(7):482-486(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press

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Anticorps Primaires

N° du Produit Hôte Isotype Clone Presentation Reactivité Applications  

DNA ligase 1 anticorps

Immunohistochemical detection of DNA Ligase I on paraffin embedded section of a human breast cancer with AM26565AF-N. Mouse IgG1 5H5 Azide Free Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 mg / 370,00 €
  Acris Antibodies GmbH

DNA ligase 1 anticorps

  Goat Anti-LIG1 / DNA ligase I Antibody  
TA305925 (0.1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Rt WB
0.1 mg / 325,00 €
  OriGene Technologies, Inc.

DNA ligase 1 anticorps

  LIG1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using LIG1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / 325,00 €
  OriGene Technologies, Inc.

DNA ligase 1 anticorps

  Rabbit Polyclonal Anti-LIG1 Antibody  
WB Suggested Anti-LIG1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysateLIG1 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb WB
50 µg / 325,00 €
  OriGene Technologies, Inc.

DNA ligase 1 anticorps

  Rabbit Polyclonal Anti-LIG1 Antibody  
WB Suggested Anti-LIG1 Antibody Titration: 0.2-1 ug/ml; Positive Control: A549 cell lysateLIG1 is supported by BioGPS gene expression data to be expressed in A549 Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / 325,00 €
  OriGene Technologies, Inc.

DNA ligase 1 anticorps

  Rabbit Polyclonal Anti-LIG1 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using (LIG1 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / 325,00 €
  OriGene Technologies, Inc.

DNA ligase 1 anticorps

Detection limit for recombinant GST tagged LIG1 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 10G12 Purified Hu E, P, WB
50 µg / 530,00 €
  Acris Antibodies GmbH

DNA ligase 1 anticorps

Immunohistochemistry (IHC) analyzes of DNA Ligase I antibody (Cat.-No.: AP20546PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / 350,00 €
  Acris Antibodies GmbH

DNA ligase 1 (N-term) anticorps

Western blot analysis of DNA liage 1 Antibody (N-term) (Cat.-No AP52477PU-N in CEM cell line lysates (35ug/lane). Rabbit Ig Aff - Purified Hu WB
0.4 ml / 370,00 €
  Acris Antibodies GmbH

DNA ligase 1 anticorps

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DNL1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
50 µg / 230,00 €
  Assay Biotech

DNA ligase 1 anticorps

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DNL1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / 330,00 €
  Assay Biotech

CRISPR

N° du Produit Espèces Donor Vector  

LIG1 - human gene knockout kit via CRISPR

LIG1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / 1 200,00 €
  OriGene Technologies, Inc.

LIG1 - human gene knockout kit via CRISPR

LIG1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / 1 290,00 €
  OriGene Technologies, Inc.

LIG1 - human gene knockout kit via CRISPR

LIG1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / 1 290,00 €
  OriGene Technologies, Inc.

LIG1 - human gene knockout kit via CRISPR

LIG1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / 1 290,00 €
  OriGene Technologies, Inc.

Lig1 - mouse gene knockout kit via CRISPR

Lig1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / 1 200,00 €
  OriGene Technologies, Inc.

Lig1 - mouse gene knockout kit via CRISPR

Lig1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / 1 290,00 €
  OriGene Technologies, Inc.

Lig1 - mouse gene knockout kit via CRISPR

Lig1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / 1 290,00 €
  OriGene Technologies, Inc.

Lig1 - mouse gene knockout kit via CRISPR

Lig1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / 1 290,00 €
  OriGene Technologies, Inc.

Proteins & Growth Factors

N° du Produit Espèces Presentation Pureté Source  

DNA ligase 1

  Recombinant protein of human ligase I, DNA, ATP-dependent (LIG1)  
DNA ligase 1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Prix normal 20 µg / 680,00 €

Prix Spécial: 20 µg / 398,00 €

  OriGene Technologies, Inc.

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