ALDH3 antibody
Principal name
ALDH3 antibody
Alternative names for ALDH3 antibody
ALDH3A1, Aldehyde dehydrogenase 3, ALDHIII, Aldehyde dehydrogenase, dimeric NADP-preferring, Aldehyde dehydrogenase family 3 member A1
Ncbi ID
9606, AAH04370, NP_000682.3, NP_001128639, NM_001135167, NM_001135168, NP_000682, NP_001128640, 10090, NM_031972, NM_000691
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ALDH3 antibody
Aldh3A1 is a member of the aldehyde dehydrogenase superfamily, a group of NAD(P)(+)-dependent enzymes that catalyze the oxidation of a wide spectrum of aliphatic and aromatic aldehydes. Aldh3A1 is highly expressed in stomach and even more strongly in cornea, representing between 5 to 50% of the water soluble protein fraction in mammalian corneas. It is thought that Aldh3A1 acts to protect the cornea from UV-induced oxidative stress by not only detoxification of reactive aldehydes by also through the direct absorbtion of UV energy. However, corneas from Aldh3A1-null mice are indistinguishable from those from wild-type mice; mice lacking both Aldh3A1 and Aldh1A1 showed increased cataract formation following UVB exposure, suggesting that Aldh1A1 may be able to compensate for the loss of Aldh3A1. At least two isoforms of Aldh3A1 are known to exist. This antibody is predicted to have no cross-reactivity to Aldh3A2.
General readings
Estey T., et al. (2007) Exp Eye Res. 84(1):3-12
Bogucka M., et al. (2009) Acta Pol Pharm. 66(5):477-82.
Detection limit for recombinant GST tagged ALDH3A1 is approximately 1ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH3 polyclonal antibody ( Cat # PAB3078 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of ALDH3 ( arrow ) using rabbit ALDH3 polyclonal antibody ( Cat # PAB3078 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the ALDH3 gene ( Lane 2 ) ( Origene Technologies ) .
ALDH3A1 MaxPab polyclonal antibody. Western Blot analysis of ALDH3A1 expression in human stomach.
Western Blot analysis of ALDH3A1 expression in transfected 293T cell line (H00000218-T01) by ALDH3A1 MaxPab polyclonal antibody.Lane 1: ALDH3A1 transfected lysate(49.83 KDa).Lane 2: Non-transfected lysate.
ALDH3A1 MaxPab polyclonal antibody. Western Blot analysis of ALDH3A1 expression in A-431.
Western Blot analysis of ALDH3A1 expression in transfected 293T cell line (H00000218-T01) by ALDH3A1 MaxPab polyclonal antibody.Lane 1: ALDH3A1 transfected lysate(50.40 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of ALDH3A1 (arrow) using rabbit polyclonal ALDH3A1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH3A1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDH3A1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
(LEFT)Western blot analysis of anti-ALDH3A1 Antibody (Center) in Y79 cell line lysates (35ug/lane). ALDH3A1(arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of anti-ALDH3A1 Antibody (Center) in mouse muscle tissue lysates (35ug/lane). ALDH3A1(arrow) was detected using the purified Pab.
Recombinant human ALDH3A1, 1-453 aa, His-tagged: 15% SDS-PAGE (3 µg)
Human Cornea; . Sample Type: Human Cornea. Dilution:. 1:200; ALDH3A1 antibody - C-terminal region (ARP59747_P050) in Human Cornea cells using Immunohistochemistry
Human Liver; WB Suggested Anti-ALDH3A1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Liver; ALDH3A1 antibody - C-terminal region (ARP59747_P050) in Human Liver cells using Western Blot
Human Cornea; . Sample Type: Human Cornea. Dilution:. 1:200; ALDH3A1 antibody - N-terminal region (ARP59748_P050) in Human Cornea cells using Immunohistochemistry
Human Fetal Muscle; ALDH3A1 antibody - N-terminal region (ARP59748_P050) validated by WB using Fetal Muscle cell lysate at 1ug/ml.; ALDH3A1 antibody - N-terminal region (ARP59748_P050) in Human Fetal Muscle cells using Western Blot
human primary hepatocytes; WB Suggested Anti-ALDH3A1 Antibody . . Positive Control: Lane1: 30ug human primary hepatocytes . Primary Antibody Dilution : 1:1000. Secondary Antibody : Anti-rabbit-HRP . Secondry Antibody Dilution : 1:5000. Submitted by: Takeshi Saito, USC Keck School of Medicine; ALDH3A1 antibody - N-terminal region (ARP59748_P050) in human primary hepatocytes cells using Western Blot
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of Aldh3A1 in human stomach lysate with Aldh3A1 antibody (AP22952PU-N) at (A) 1 and (B) 2 µg/ml.
Western blot analysis of ALDH3A1 expression in A549 (A) whole cell lysates.
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501105, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501105, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501105, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501105, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501105, Dilution 1:50)
Anti-ALDH3A1 mouse monoclonal antibody (TA501105) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH3A1(RC202440).
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ALDH3A1 monoclonal antibody at 1:200 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH3A1 (RC202440, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A1.
HEK293T cells transfected with either pCMV6-ENTRY ALDH3A1(RC202440)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ALDH3A1 mouse monoclonal(TA501105), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501139, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501139, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501139, Dilution 1:50)
Anti-ALDH3A1 mouse monoclonal antibody (TA501139) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH3A1(RC202440).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH3A1 (RC202440, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A1.
HEK293T cells transfected with either pCMV6-ENTRY ALDH3A1(RC202440)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ALDH3A1 mouse monoclonal(TA501139), and then analyzed by flow cytometry.
Western blot analysis of Aldh3A1 in human stomach lysate with Aldh3A1 antibody at (A) 1 and (B) 2 µg/ml.
(0.01ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA311135 (3.8µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Predicted band size: 50 kDa . Positive control: A549 cell and mouse eye tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Mouse eye tissue lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ALDH3A1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted band size: 50 kDa . Positive control: A549 cell and mouse eye tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Mouse eye tissue lysate Lysates: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm. Positive control: Human gastric cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using ALDH3A1 antibody at dilution 1/55, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis of extracts of various cell lines, using ALDH3A1 antibody.
Immunohistochemistry of paraffin-embedded human thyroid cancer using ALDH3A1 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded rat lung using ALDH3A1 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of U20S cell using ALDH3A1 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-ALDH3A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver
Sample Type: Human Cornea; Dilution: 1:200
ALDH3A1 antibody validated by WB using Fetal Muscle cell lysate at 1 ug/ml.
WB Suggested Anti-ALDH3A1 Antibody; Positive Control: Lane1: 30ug human primary hepatocytes; Primary Antibody Dilution: 1:1000; Secondary Antibody: Anti-rabbit-HRP; Secondry Antibody Dilution: 1:5000; Submitted by: Takeshi Saito, USC Keck School of Medici
Sample Type: Human Cornea; Dilution: 1:200
- Filtre rapide:
- Validés (3)
Anticorps Primaires
| N° du Produit | Hôte | Isotype | Clone | Presentation | Reactivité | Applications | |
|---|---|---|---|---|---|---|---|
| TA332730 | ALDH3 anticorps |
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| Rabbit anti-ALDH3A1 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
335,00 €
|
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| OriGene Technologies, Inc. | |||||||
 +3 Images additionnelles |
Highly validated antibody! |
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| TA501105 | ALDH3 anticorps |
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| anti-ALDH3A1 mouse monoclonal antibody, clone OTI1B6 (formerly 1B6) | |||||||
 |
Mouse | IgG2a | OTI1B6 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
379,00 €
|
| OriGene Technologies, Inc. | |||||||
| +8 Images additionnelles |
Highly validated antibody!
|
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| TA501139 | ALDH3 anticorps |
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| anti-ALDH3A1 mouse monoclonal antibody, clone OTI1C6 (formerly 1C6) | |||||||
 |
Mouse | IgG2a | OTI1C6 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
350,00 €
|
| OriGene Technologies, Inc. | |||||||
| +6 Images additionnelles |
Highly validated antibody!
|
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