AR09050PU-L Interleukin-15 / IL15 (49-162, His-tag)

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0.5 mg / 1 070,00 €
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Interleukin-15 / IL15

AR09050PU-L

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  • AR09050PU-L

Description du Produit Interleukin-15 / IL15

Interleukin-15 / IL15.
Presentation: Purified

Caractéristiques du Produit Interleukin-15 / IL15

Type de Produit Proteins & Growth Factors
Target Category
Quantité 0.5 mg
Synonymes IL-15
Presentation Purified
Poids Moléculaire 13.9 kDa
Source E. coli
Espèce (Protéine) Human
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Proteins (de)

Extrait de la Fiche Technique

Immunogène
Swiss Prot:
P40933
Pureté > 95 % by SDS PAGE
Sujet Interleukin (IL-15) is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma(c)-expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells.
Concentration 1.0 mg/ml
Références Générales Lorenzen I., et al., (2006). J Biol Chem. Mar 10; 281 (10):6642-7.
Stockage Store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human IL-15, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.
Séquence AA:
MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTSLEHHH HHH
Biological activity:
Measured in a cell proliferation assay using CTLL2 mouse cytotoxic
T cells. The ED50 for this effect is < 2.5 ng/ml.
Activity Assay
1.Cell line: CTLL2 (mouse cytotoxic T cell)
2.Maintenance Condition: 10% FBS RPMI 1640 with hIL2
3.Assay Medium: 10% FBS RPMI 1640 without hIL2
4.Cell Density: 2 x 10e4 cells/well (96 well plate, final volume 100ul)
5.Starvation : 24hr, Assay medium
6.Incubation Time: 24hr (after sample treatment)
7.Concentration Range: 0.039 ng/ml - 40 ng/ml
8.Detection Method : MTT assay
Poids Moléculaire:
(123 aa), confirmed by MALDI-TOF
Presentation
Buffer:
PBS, pH 7.4, 0.2 mM PMSF, 10% glycerol
Endotoxin level:
< 1.0 EU per 1 μg of protein (determined by LAL method)
Pureté:
>95% by SDS PAGE
Format:
Liquid purified protein
Purified
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