AR50084PU-S Intein (3-518, His-tag)

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0.1 mg / 300,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Vue générale rapide

Intein

Description du Produit Intein

Intein.
Presentation: Purified

Caractéristiques du Produit Intein

Type de Produit Proteins & Growth Factors
Quantité 0.1 mg
Synonymes Intein-CBD
Presentation Purified
Poids Moléculaire 59.4 kDa
Source E. coli
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Proteins (de)

Extrait de la Fiche Technique

Pureté > 95 % by SDS - PAGE
Sujet Intein-CBD, also known as Intein, is a segment of a protein that is able to excise itself and rejoin the remaining portions with a peptide bond. Most reported inteins also contain an endonuclease domain that plays a role in intein propagation. In fact, many genes have unrelated intein-coding segments inserted at different positions. Since then, inteins have been found in all three domains of life (eukaryotes, bacteria, and archaea) and in viruses.
Concentration 1 mg/ml (determined by Bradford assay)
Références Générales
  1. Goqarten JP (2006) BMC Evol Biol. 13:6-94.
  2. Anraku Y, Mizutani R, Satow Y. Protein splicing: its discovery and structural insight into novel chemical mechanisms. IUBMB Life. 2005 Aug;57(8):563-74. PubMed PMID: 16118114.
Stockage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Bacillus circulans Intein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. 
N-terminal Sequence Analysist: Met-Gly-Pro-Thr-Gly
Séquence AA:
MKIEEGKLVI GSLEGCFAKG TNVLMADGSI ECIENIEVGN KVMGKDGRPR EVIKLPRGRE TMYSVVQKSQ HRAHKSDSSR EVPELLKFTC NATHELVVRT PRSVRRLSRT IKGVEYFEVI TFEMGQKKAP DGRIVELVKE VSKSYPISEG PERANELVES YRKASNKAYF EWTIEARDLS LLGSHVRKAT YQTYAPILYE NDHFFDYMQK SKFHLTIEGP KVLAYLLGLW IGDGLSDRAT FSVDSRDTSL MERVTEYAEK LNLCAEYKDR KEPQVAKTVN LYSKVVRGAS TNPGVSAWQV NTAYTAGQLV TYNGKTYKCL QPHTSLAGWE PSNVPALWQL QGGHGGIRNN LNTENPLWDA IVGLGFLKDG VKNIPSFLST DNIGTRETFL AGLIDSDGYV TDEHGIKATI KTIHTSVRDG LVSLARSLGL VVSVNAEPAK VDMNVTKHKI SYAIYMSGGD VLLNVLSKCA GSKKFRPAPA AAFARECRGF YFELQELKED DYYGITLSDD SDHQFLLGSQ VVVQNLEHHH HHH
Poids Moléculaire:
(533aa), confirmed by MALDI-TOF
Presentation
Buffer:
20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Pureté:
>95% by SDS - PAGE
Format:
Liquid purified protein
Purified
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