AR51950PU-N gldA (1-367, His-tag)

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0.1 mg / 370,00 €
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Vue générale rapide

gldA

Description du Produit gldA

gldA.
Presentation: Purified

Caractéristiques du Produit gldA

Type de Produit Proteins & Growth Factors
Quantité 0.1 mg
Synonymes ECK3937, Glycerol dehydrogenase, JW5556
Presentation Purified
Poids Moléculaire 41.1 kDa
Source E. coli
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Proteins (de)

Extrait de la Fiche Technique

Immunogène
Swiss Prot:
P0A9S5
Pureté > 95 % by SDS - PAGE
Sujet gldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E.coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i.e. the conversion of dihydroxyacetone into glycerol. gldA possesses a broad substrate specificity, since it is also able to oxidize 1,2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1,2-propanediol, respectively.
Concentration 1.0 mg/ml (determined by bradford assay)
Protocols 1. Prepare a 200ul reaction mix into a suitable container: The final concentrations are 93mM Glycine, 93mM Potassium chloride, 2375mM Glycerol, 3mM b-NAD.

2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.

3. Add 20ul of recombinant gldA protein with various concentrations (0.2ug, 0.1ug, 0.05ug) in 180ul reaction buffer.

4. Mix by inversion and record the decrease at A340nm for 10 minutes.

Références Générales Subedi K.P., et al. (2008) FEMS Microbiol. Lett. 279:180-187Gonzalez R., et al. (2008) Metab. Eng. 10:234-245
Stockage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E. coli gldA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Séquence AA:
MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE
Poids Moléculaire:
(390aa), confirmed by MALDI-TOF
Specific activity:
> 14 Units/ml One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25C
Presentation
Buffer:
Phosphate buffered saline (pH7.4), 10% glycerol.
Pureté:
>95% by SDS - PAGE
Format:
Liquid purified protein
Purified
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