AR09371PU-L ALDH3 (1-453, His-tag)

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0.25 mg / 1 000,00 €
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ALDH3

AR09371PU-L

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  • AR09371PU-L

Description du Produit ALDH3

ALDH3.
Presentation: Purified

Caractéristiques du Produit ALDH3

Type de Produit Proteins & Growth Factors
Target Category
Quantité 0.25 mg
Synonymes ALDH3A1, ALDHIII, Aldehyde dehydrogenase, Aldehyde dehydrogenase 3, Aldehyde dehydrogenase family 3 member A1, dimeric NADP-preferring
Presentation Purified
Poids Moléculaire 52.5 kDa
Source E. coli
Espèce (Protéine) Human
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur Acris Antibodies GmbH
Material Safety DataSheet (MSDS) MSDS for Proteins (de)

Extrait de la Fiche Technique

Immunogène
Swiss Prot:
P30838
Pureté > 95 % by SDS - PAGE
Sujet ALDH3A1, also known as Aldehyde dehydrogenase 3 family memberA1, is involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea.
Concentration 0.5 mg/ml (determined by Bradford assay)
Références Générales Estey T., et al. (2007) Exp Eye Res. 84(1):3-12
Bogucka M., et al. (2009) Acta Pol Pharm. 66(5):477-82.
Stockage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant ALDH3A1 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
Séquence AA:
MGSSHHHHHH SSGLVPRGSH MSKISEAVKR ARAAFSSGRT RPLQFRIQQL EALQRLIQEQ EQELVGALAA DLHKNEWNAY YEEVVYVLEE IEYMIQKLPE WAADEPVEKT PQTQQDELYI HSEPLGVVLV IGTWNYPFNL TIQPMVGAIA AGNAVVLKPS ELSENMASLL ATIIPQYLDK DLYPVINGGV PETTELLKER FDHILYTGST GVGKIIMTAA AKHLTPVTLE LGGKSPCYVD KNCDLDVACR RIAWGKFMNS GQTCVAPDYI LCDPSIQNQI VEKLKKSLKE FYGEDAKKSR DYGRIISARH FQRVMGLIEG QKVAYGGTGD AATRYIAPTI LTDVDPQSPV MQEEIFGPVL PIVCVRSLEE AIQFINQREK PLALYMFSSN DKVIKKMIAE TSSGGVAAND VIVHITLHSL PFGGVGNSGM GSYHGKKSFE TFSHRRSCLV RPLMNDEGLK VRYPPSPAKM TQH
Biological activity:
Specific activity is < 1 unit/ml and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD.
One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25°C in the presence of beta-NAD, potassium and thiols.


Activity Assay
1. Prepare a 3 ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA.
2. Equilibrate to 25°C and monitor the A340nm until the value is constant using a spectrophotometer.
3. Add 2.5 ug of recombinant ALDH3A1 into reaction mixture and mix immediately.
4. Record the increase in A340nm for 5 minutes.
Poids Moléculaire:
(473 aa)
Presentation
Buffer:
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1 M NaCl
Pureté:
>95% by SDS - PAGE
Format:
Liquid purified protein
Purified
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