Caractéristiques du Produit Ovary Lysate

Type de Produit Lysates
Quantité 0.1 mg
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH

Extrait de la Fiche Technique

Applications Western blot: 10 μg to 20 μg per lane is recommended for mini gel.
Tissue Lysate Preparation:
Human Ovary tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration 2.0 mg/ml
Stockage Store at -20°C for 3 months or at -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
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