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CPK2132-200rxns hsa-mir-194 Real-Time RT-PCR Detection and U6 Calibration Kit

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CPK2132-200rxns

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  • CPK2132-200rxns
  • CPK2132-200rxns
  • CPK2132-200rxns

Caractéristiques du Produit hsa-mir-194 Real-Time RT-PCR Detection and U6 Calibration Kit

Type de Produit PCR Kits
Quantité 200 rxns
Reactivité d'Espèce Hu
Destination Europe only
Fiche Technique Télécharger la fiche technique
Fournisseur Cohesion Biosciences Ltd.

Extrait de la Fiche Technique

Applications Real-time RT-PCR
The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
Description: Detect mature miRNA hsa-mir-194 expression normalized by U6 snRNA.
PCR Method: 2-Step qRT-PCR
Detection Range: Seven orders of magnitude
Sensitivity: 10 copies of a standard mimics and as little as 25 pg of total RNA
Sample Types: miRNA, Total RNA
Platform: ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480
Components: 50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.
Stockage Shipped at 4°C. Store at -20°C for 6 months.
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