BB-EK0356 Human Fractalkine/CX3CL1 ELISA Kit

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Caractéristiques du Produit Human Fractalkine/CX3CL1 ELISA Kit

Type de Produit ELISA Kits
Quantité 96 Tests
Reactivité d'Espèce Hu
Destination Not USA/Canada
Fiche Technique Télécharger la fiche technique
Fournisseur Boster Immunoleader
Manufacturer certificates ISO 9001

Extrait de la Fiche Technique

Swiss Prot:
Add. information Sensitivity < 50 pg/ml
Applications Sandwich ELISA kit of Quantitative Detection for Human Fractalkine/CX3CL1
This kit is used for quantitative detection of human Fractalkine in cell culture supernates, serum and plasma(heparin, EDTA), saliva, urine and human milk.
There is detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC < 0.2%.
Sujet Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice).1, 2 CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.2 CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1.3 Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22.2, 4 It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.5
Références Générales
  1. Pan Y, Lloyd C, Zhou H, Dolich S, Deeds J, Gonzalo JA, et al. Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation. Nature. 1997 Jun 5;387(6633):611-7. PubMed PMID: 9177350.
  2. Bazan JF, Bacon KB, Hardiman G, Wang W, Soo K, Rossi D, et al. A new class of membrane-bound chemokine with a CX3C motif. Nature. 1997 Feb 13;385(6617):640-4. PubMed PMID: 9024663.
  3. Imai T, Hieshima K, Haskell C, Baba M, Nagira M, Nishimura M, et al. Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell. 1997 Nov 14;91(4):521-30. PubMed PMID: 9390561.
  4. Nomiyama H, Imai T, Kusuda J, Miura R, Callen DF, Yoshie O. Human chemokines fractalkine (SCYD1), MDC (SCYA22) and TARC (SCYA17) are clustered on chromosome 16q13. Cytogenet Cell Genet. 1998;81(1):10-1. PubMed PMID: 9691168.
  5. Lucas AD, Bursill C, Guzik TJ, Sadowski J, Channon KM, Greaves DR. Smooth muscle cells in human atherosclerotic plaques express the fractalkine receptor CX3CR1 and undergo chemotaxis to the CX3C chemokine fractalkine (CX3CL1). Circulation. 2003 Nov 18;108(20):2498-504. Epub 2003 Oct 27. PubMed PMID: 14581400.
Stockage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Range 156-10,000 pg/ml
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