R1427R Rabbit IgG F(ab)2 Fragment (H+L chain) anticorps

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0.5 mg / 270,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

R1427R

Description du Produit Rabbit IgG

Properties: F(ab)2 Fragment (H+L chain)
Presentation: PE
Product is tested for Flow Cytometry.

Caractéristiques du Produit Rabbit IgG

Type de Produit Anticorps Secondaires
Target Category
Quantité 0.5 mg
Synonymes Rabbit Immunoglobulin G
Presentation PE
Reactivité d'Espèce Rb
Applications F
Clonalité Polyclonal
Hôte Donkey
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Rabbit IgG whole molecule.
Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
Immunofluorescence: 1/100-1/200.
Flow Cytometry: 1/100-1/20. The maximum amount of reagent required to stain 1 x 10e6 cells is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Concentration 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm)
Stockage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Presentation
Conjugation:
R-Phycoerythrin ( Molecular Weight 240,000 daltons)
PE
Absorption emission:
488 nm / 575 nm
Purification:
Immunoaffinity Chromatography.
Buffer:
0.02M Potassium Phosphate, 0.15M Sodium Chloride; pH 7.2
Agent Conservateur:
0.01% (w/v) Sodium Azide
Stabilisateurs:
10 mg/ml BSA (Immunoglobulin and Protease free)
Format:
Lyophilized F(ab')2 fragments.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Reactivité d'Espèce
Reactivité d'Espèce (Testé):
Rabbit.
Specificité
Specificité:
This product was prepared from monospecific antiserum by This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.
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