R1364HRP Rabbit IgG (H+L chain) anticorps

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2 mg / 220,00 €
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Description du Produit Rabbit IgG

Properties: (H+L chain)
Presentation: HRP
Product is tested for Paraffin Sections, Western blot / Immunoblot, Enzyme Immunoassay.

Caractéristiques du Produit Rabbit IgG

Type de Produit Anticorps Secondaires
Target Category
Quantité 2 mg
Synonymes Rabbit Immunoglobulin G
Presentation HRP
Reactivité d'Espèce Rb
Applications E, P, WB
Clonalité Polyclonal
Hôte Goat
Poids Moléculaire 40 kDa
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur Acris Antibodies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Rabbit IgG, whole molecule
Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/150,000-1/250,000.
Western blot: 1/10,000-1/20,000.
Immunohistochemistry: 1/500-1/2,500.
Sujet Rabbit secondary antibody conjugated to horseradish peroxidase (HRP) is available in a variety of formats. HRP Anti IgG secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western Blotting as well as other HRP antibody based assays.
Caution Do Not Add Sodium Azide.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
  1. Kokk K, Kuuslahti M, Keisala T, Purmonen S, Kaipia A, Tammela T, et al. Expression of luteinizing hormone receptors in the mouse penis. J Androl. 2011 Jan-Feb;32(1):49-54. doi: 10.2164/jandrol.109.008623. Epub 2010 Aug 26. PubMed PMID: 20798391.
Références Générales
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144, 1981. (Conjugation)
Stockage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Presentation
Conjugation:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads
Buffer:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Agent Conservateur:
0.01% (w/v) Gentamicin Sulfate
Stabilisateurs:
10 mg/ml BSA (Ig and Protease free)
Format:
Lyophilized purified Ig fraction
Lyophilized
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificité
Specificité:
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
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