R1253HRP Mouse IgG (H+L chain) anticorps

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2 mg / 230,00 €
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Description du Produit Mouse IgG

Properties: (H+L chain)
Presentation: HRP
Product is tested for Frozen Sections, Western blot / Immunoblot, Enzyme Immunoassay.

Caractéristiques du Produit Mouse IgG

Type de Produit Anticorps Secondaires
Target Category
Quantité 2 mg
Synonymes Mouse Immunoglobulin G
Presentation HRP
Applications C, E, WB
Clonalité Polyclonal
Hôte Rabbit
Poids Moléculaire 40,000 Da
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur Acris Antibodies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Mouse IgG whole molecule.
Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA : 1:50,000 - 1:300,000
Western Blot : 1:10,000 - 1:30,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Caution Do Not Add Sodium Azide.
Concentration 2.0 mg/ml (by UV absorbance at 280nm)
Références Générales
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation).
  2. Sone H, Kagawa Y. Pancreatic beta cell senescence contributes to the pathogenesis of type 2 diabetes in high-fat diet-induced diabetic mice. Diabetologia. 2005 Jan;48(1):58-67. Epub 2004 Dec 29. PubMed PMID: 15624098.
Stockage Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot and freeze at -20°C or below.
Avoid cycles of freezing and thawing! Dilute only prior to immediate use!

Presentation
Conjugation:
Horseradish Peroxidase
Conjugation Chemistry: Periodate
HRP
Purification:
Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads
Buffer:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Agent Conservateur:
0.01% (w/v) Gentamicin Sulfate
Stabilisateurs:
10 mg/ml BSA (IgG and Protease free)
Pureté:
and
Format:
Lyophilized purified Ig fraction
Reconstitution:
Reconstitute with 1.0 ml of deionized water (or equivalent).
Specificité
Specificité:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
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