R1253B Mouse IgG (H+L chain) anticorps

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2 mg / 230,00 €
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R1253B

Description du Produit Mouse IgG

Properties: (H+L chain)
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Caractéristiques du Produit Mouse IgG

Type de Produit Anticorps Secondaires
Target Category
Quantité 2 mg
Synonymes Mouse Immunoglobulin G
Presentation Biotin
Applications C, E, WB
Clonalité Polyclonal
Hôte Rabbit
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur Acris Antibodies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Mouse IgG whole molecule.
Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Références Générales
  1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Stockage Store secondary antibody at 4° C prior to reconstitution.  
Centrifuge product if not completely clear after standing at room temperature. 
This product is stable for several weeks at 4° C as an undiluted liquid. 
Dilute only prior to immediate use. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing!
Presentation
Conjugation:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Rabbit IgG molecule
Purification:
Immunoaffinity Chromatography
Buffer:
0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4
Agent Conservateur:
0.01% (w/v) Sodium Azide
Stabilisateurs:
10 mg/ml BSA (IgG and Protease free)
Pureté:
and
Format:
Lyohilized purified Ig fraction
Reconstitution:
Reconstitute  with 1.0 ml of deionized water (or equivalent).
Specificité
Specificité:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
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