discontinued

R1435C5 Goat IgG (H+L chain), adsorbed anticorps

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R1435C5

Description du Produit Goat IgG

Properties: (H+L chain), adsorbed
Presentation: Cy5
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Caractéristiques du Produit Goat IgG

Type de Produit Anticorps Secondaires
Target Category
Quantité 1 mg
Synonymes Goat Immunoglobulin G
Presentation Cy5
Applications F, ICC/IF
Clonalité Polyclonal
Hôte Donkey
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur Acris Antibodies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Goat IgG whole molecule.
Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
Immunofluorescence: 1/100-1/1,000.
Flow Cytometry: 1/200-1/400.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Références Générales
  1. Modified from GE Healthcare Bio-Sciences Corp., Piscataway, NJ.
Stockage Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Presentation
Conjugation:
29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons)
Cy5
Absorption emission:
650 nm / 667 nm
Molar radio:
12.5 moles Cy5 per mole of Donkey IgG.
Purification:
Immunoaffinity Chromatography.
Buffer:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 and conjugated to Cy5.29 (Cyanine 5.29-OSu) containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
Format:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificité
Specificité:
This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum.
No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Sheep Serum Proteins.
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