R1416R Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed anticorps

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0.5 mg / 380,00 €
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R1416R

Description du Produit Goat IgG

Properties: (H+L chain), F(ab)2 Fragment, adsorbed
Presentation: PE

Caractéristiques du Produit Goat IgG

Type de Produit Anticorps Secondaires
Target Category
Quantité 0.5 mg
Synonymes Goat Immunoglobulin G
Presentation PE
Clonalité Polyclonal
Hôte Donkey
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur Acris Antibodies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Goat IgG whole molecule
Applications Suitable for Immunomicroscopy (1/100-1/200) and Flow Cytometry (1/100-1/200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Concentration 0.65 mg/ml (determined by absorbance at 565 nm for 0.1% Solution)
Références Générales
  1. Carlsson J, Drevin H, Axén R. Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent. Biochem J. 1978 Sep 1;173(3):723-37. PubMed PMID: 708370. (Free PMC Article available)
Stockage Store the antibody undiluted at 2-8°C prior to opening.
DO NOT FREEZE!
Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
Presentation
Conjugation:
R-Phycoerythrin (( from seaweed; Molecular Weight 240,000 daltons). Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those molecules to which at least one molecule of active antibody is bound)
PE
Absorption emission:
488/575 nm
Purification:
Immunoaffinity Chromatography.
Buffer:
0.02M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and protease free), 0.05% (w/v) Sodium Azide as preservative.
Pureté:
/
Format:
Lyophilized F(ab')2 fragments.
Reconstitution:
Restore with 1 ml distilled sterile water.
Specificité
Specificité:
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum.
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, or Rat Serum Proteins or other Goat heavy or light chain proteins.
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