R1317B Goat IgG (H+L chain) anticorps

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2 mg / 210,00 €
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R1317B

Description du Produit Goat IgG

Properties: (H+L chain)
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Caractéristiques du Produit Goat IgG

Type de Produit Anticorps Secondaires
Target Category
Quantité 2 mg
Synonymes Goat Immunoglobulin G
Presentation Biotin
Applications C, E, WB
Clonalité Polyclonal
Hôte Rabbit
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur Acris Antibodies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Goat IgG whole molecule.
Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other assays using streptavidin/avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/1,00,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Références Générales
  1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Stockage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Presentation
Conjugation:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Rabbit IgG molecule.
Purification:
Immunoaffinity Chromatography
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Agent Conservateur:
0.01% (w/v) Sodium Azide
Stabilisateurs:
10 mg/ml BSA (IgG and Protease free)
Format:
Lyophilized purified Ig fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificité
Specificité:
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG and Goat Serum.
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