R1616B Bovine IgG (H+L chain) anticorps

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2 mg / 210,00 €
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R1616B

Description du Produit Bovine IgG

Properties: (H+L chain)
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Caractéristiques du Produit Bovine IgG

Type de Produit Anticorps Secondaires
Target Category
Quantité 2 mg
Synonymes Bovine Immunoglobulin G
Presentation Biotin
Reactivité d'Espèce Bov
Applications C, E, P, WB
Clonalité Polyclonal
Hôte Goat
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Bovine IgG whole molecule.
Applications Suitable for Immunoblotting (1:2,000-1:10,000), ELISA (see below), Immunohistochemistry (1:1000-1:5000), Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:11,000 to 1:45,000 of the reconstitution concentration is suggested for this product.
Concentration 2.0 mg/ml (before lyophil. by UV absorbance at 280 nm)
Références Générales
  1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Stockage Store vial at 2-8°C prior to restoration.
Restore with 1.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: one year from despatch.
Presentation
Conjugation:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Goat IgG molecule.
Purification:
Immunoaffinity chromatography.
Buffer:
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 contaning 0.01% (w/v) Thimerisol as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
Format:
Lyophilized purified IgG fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificité
Specificité:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against antibiotin, anti-Goat Serum, Bovine IgG and Bovine Serum.
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