R1306HRP Bovine IgG (F(ab)2 specific) anticorps

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2 mg / 290,00 €
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R1306HRP

Description du Produit Bovine IgG

Properties: (F(ab)2 specific)
Presentation: HRP
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Caractéristiques du Produit Bovine IgG

Type de Produit Anticorps Secondaires
Target Category
Quantité 2 mg
Synonymes Bovine Immunoglobulin G
Presentation HRP
Reactivité d'Espèce Bov
Applications C, E, ICC/IF, WB
Clonalité Polyclonal
Hôte Rabbit
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Bovine IgG F(ab')2 fragment.
Applications Suitable for Immunoblotting (1:1,000-10,000), ELISA (1:10,000 - 1:50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Caution Do Not Use Sodium Azide as Preservative.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Références Générales Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Stockage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Presentation
Conjugation:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity chromatography.
Buffer:
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% Gentamicin Sulfate as preservative.
Format:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificité
Specificité:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(ab')2 and Bovine Serum.
No reaction was observed against Bovine IgG F(c).
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