R1305B Bovine IgG (Fc specific) anticorps

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1.5 mg / 280,00 €
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R1305B

Description du Produit Bovine IgG

Properties: (Fc specific)
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Caractéristiques du Produit Bovine IgG

Type de Produit Anticorps Secondaires
Target Category
Quantité 1.5 mg
Synonymes Bovine Immunoglobulin G
Presentation Biotin
Reactivité d'Espèce Bov
Applications C, E, ICC/IF, WB
Clonalité Polyclonal
Hôte Rabbit
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Bovine IgG F(c) fragment.
Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/15,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Concentration 1.5 mg/ml (by UV absorbance at 280 nm)
Références Générales Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation).
Stockage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Presentation
Conjugation:
Biotin
Molar radio:
10-20 BAC molecules per Rabbit IgG molecule.
Purification:
Immunoaffinity chromatography.
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
Format:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificité
Specificité:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum.
No reaction was observed against Bovine IgG F(ab')2.
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