AM33341PU-S TAG72 anticorps

See related secondary antibodies

Search for all "TAG72"

0.1 mg / 320,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Vue générale rapide

Mouse anti Bovine, Canine, Hamster, Human, Rat TAG72 B72.3 + CA72/733

Description du Produit TAG72

Mouse anti Bovine, Canine, Hamster, Human, Rat TAG72 B72.3 + CA72/733.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Frozen Sections, Paraffin Sections.

Caractéristiques du Produit TAG72

Type de Produit Anticorps Primaires
Quantité 0.1 mg
Synonymes CA 72-4, CA72-4, Tumor-Associated Glycoprotein 72, sialy-Tn
Presentation Purified
Reactivité d'Espèce Bov, Can, Hst, Hu, Rt
Applications C, F, ICC/IF, IP, P, WB
Clonalité Monoclonal
Clone B72.3 + CA72/733
Hôte Mouse
Isotype IgG1
Poids Moléculaire 220kDa
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Monoclonal Antibodies (de)

Extrait de la Fiche Technique

TAG-72 protein (B72.3 & CA72/733).
Isotype Control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Applications Western Blot: 0.5-1 µg/ml. 
Flow Cytometry: 0.5-1 µg/106 cells. 
Immunoprecipitation: 1-2 µg/500 µg protein lysate. 
Immunofluorescence: 1-2 µg/ml.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. 
Positive Control: Jurkat cells, Breast or lung carcinoma.
Sujet Tumor-associated glycoprotein (TAG) 72 is a tumor marker, identified and characterized using two different monoclonal antibodies B72.3 and CC49. It is a mucin-like protein of high molecular weight (220-400 kd). The protein is expressed in several epithelial-derived cancers including 94% of colonic adenocarcinomas, 84% of invasive ductal carcinomas of the breast, 96% of non-small cell lung carcinomas, 100%of common epithelial ovarian carcinomas, as well as the majority of pancreatic, gastric, and esophageal cancers evaluated. The protein expression is also detected in fetal colon, stomach, and esophagus, thus defining TAG-72 as an oncofetal antigen.
Concentration 0.2 mg/ml
Références Générales
  1. Lottich SC, Johnston WW, Szpak CA, Delong ER, Thor A, Schlom J. Tumor-associated antigen TAG-72: correlation of expression in primary and metastatic breast carcinoma lesions. Breast Cancer Res Treat. 1985;6(1):49-56. PubMed PMID: 2996665.
  2. Thor A, Ohuchi N, Szpak CA, Johnston WW, Schlom J. Distribution of oncofetal antigen tumor-associated glycoprotein-72 defined by monoclonal antibody B72.3. Cancer Res. 1986 Jun;46(6):3118-24. PubMed PMID: 3516392.
Stockage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
10mM PBS
Agent Conservateur:
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
Reactivité d'Espèce
Reactivité d'Espèce (Testé):
Human, Cow, Dog, and Rat.
This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin.
This Monoclonal antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Cellular Localization: Cell surface and cytoplasmic.

Accessory Products

  • LinkedIn