CL077F T Cell Receptor (TCR) V beta 4 anticorps

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Rat anti Mouse T Cell Receptor (TCR) V beta 4 CTVB4

Description du Produit T Cell Receptor (TCR) V beta 4

Rat anti Mouse T Cell Receptor (TCR) V beta 4 CTVB4.
Presentation: FITC
Product is tested for Flow Cytometry.

Caractéristiques du Produit T Cell Receptor (TCR) V beta 4

Type de Produit Anticorps Primaires
Quantité 0.1 mg
Synonymes TCR V beta-4, TCR Vb4
Presentation FITC
Reactivité d'Espèce Ms
Applications F
Clonalité Monoclonal
Clone CTVB4
Hôte Rat
Isotype IgG2a
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Monoclonal Antibodies (de)

Extrait de la Fiche Technique

Applications Flow Cytometry.
Concentration 0.1 mg/ml

1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add ~1.0 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most flurochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results - Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: C3H.SW
Cell Concentration: 1x10e6 cells per tests
Antibody Concentration Used: 1.0 µg/10e6 cells
Isotypic Control: FITC Rat IgG2a
Références Générales
  1. Tomonari, K., lovering, E. and Spencer, S. 1990 Correlation between the Vb4+ CD8+ T cell population and the H-2d haplotype. Immunogenetics 31:333-339.
  2. Padula, S.J., Lingenheld, E.G., Stabach, P.R., Chou, C.J., Kono, D.H. and Clark, R.B. 1991 Identificaion of encephalitogenic Vb4-bearing T cells in SJL mice-further evidence for the V region disease hyopothesis? J. Immunol. 146:879-883.
Stockage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should protected from light.
Shelf life: one year from despatch.
PBS, 0.09% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Liquid purified Ig
This antigen monoclonal antibody reats with TCR Vb4 bearing T cells. TCR Vb4 is expressed in most known mouse strains.

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