AP21390BT-N Subtilisin anticorps

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1 ml / 390,00 €
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Rabbit anti Bacillus sp. Subtilisin


Description du Produit Subtilisin

Rabbit anti Bacillus sp. Subtilisin.
Presentation: Biotin
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Caractéristiques du Produit Subtilisin

Type de Produit Anticorps Primaires
Quantité 1 ml
Synonymes Subtilisin E, apr, aprA, aprE, sprE
Presentation Biotin
Reactivité d'Espèce Bacillus sp.
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonalité Polyclonal
Hôte Rabbit
Isotype IgG
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Swiss Prot:
Subtilisin isolated and purified from Bacillus subtilis.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Applications This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
Recommended Dilutions:
Non-precipitating antibody-binding techniques: 1/1,000-1/6,000.
Sujet Subtilisin (serine endopeptidase) is a non-specific protease (a protein-digesting enzyme) initially obtained from Bacillus subtilis.
Subtilisins belong to subtilases, a group of serine proteases that initiate the nucleophilic attack on the peptide (amide) bond through a serine residue at the active site. They are physically and chemically well-characterized enzymes. Subtilisins typically have molecular weights of about 20,000 to 45,000 dalton. They can be obtained from soil bacteria, for example, Bacillus amyloliquefaciens. Subtilisins are secreted in large amounts from many Bacillus species.
Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
Concentration 10.0 mg/ml
Stockage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Shelf life: one year from despatch.
Molar radio:
Biotin/IgG ~ 6.3
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
PBS, pH 7.2 without preservatives and foreign proteins
Lyophilized hyperimmune IgG fraction
Restore by adding 1 ml of sterile distilled water
Subtilisin from Bacillus subtilis.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Bacillus subtilis.
ID Gène 939313

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