AM05879FC-N Rituximab anticorps

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0.1 mg / 850,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rat anti Rituximab MB2 A4


Description du Produit Rituximab

Rat anti Rituximab MB2 A4.
Presentation: FITC
Product is tested for Flow Cytometry.

Caractéristiques du Produit Rituximab

Type de Produit Anticorps Primaires
Quantité 0.1 mg
Synonymes MabThera, Rituxan
Presentation FITC
Applications F
Clonalité Monoclonal
Clone MB2 A4
Hôte Rat
Isotype IgG2a
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Monoclonal Antibodies (de)

Extrait de la Fiche Technique

F(ab)2 fragment of Rituximab
Applications Flow Cytometry: Use 10 µl of neat antibody to label 106 cells in 100 µl.
Sujet Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen.
Concentration 0.1 mg/ml
Références Générales
  1. Bayne, M. et al. (2003) Phase I/II study of fractionated radioimmunotherapy in relapsed low grade non Hodgkin lymphoma. Br. J. Cancer. 88 : S38.
  2. Cragg MS, Bayne MC, Illidge TM, Valerius T, Johnson PW, Glennie MJ. Apparent modulation of CD20 by rituximab: an alternative explanation. Blood. 2004 May 15;103(10):3989-90; author reply 3990-1. PubMed PMID: 15121717.
  3. Beum PV, Kennedy AD, Li Y, Pawluczkowycz AW, Williams ME, Taylor RP. Complement activation and C3b deposition on rituximab-opsonized cells substantially blocks binding of phycoerythrin-labeled anti-mouse IgG probes to rituximab. J Immunol Methods. 2004 Nov;294(1-2):37-42. PubMed PMID: 15604014.
  4. Hampson G, Ward TH, Cummings J, Bayne M, Tutt AL, Cragg MS, et al. Validation of an ELISA for the determination of rituximab pharmacokinetics in clinical trials subjects. J Immunol Methods. 2010 Aug 31;360(1-2):30-8. doi: 10.1016/j.jim.2010.05.009. Epub 2010 Jun 11. PubMed PMID: 20547164.
  5. Blasco H, Lalmanach G, Godat E, Maurel MC, Canepa S, Belghazi M, et al. Evaluation of a peptide ELISA for the detection of rituximab in serum. J Immunol Methods. 2007 Aug 31;325(1-2):127-39. Epub 2007 Jul 17. PubMed PMID: 17651747.
  6. Daydé D, Ternant D, Ohresser M, Lerondel S, Pesnel S, Watier H, et al. Tumor burden influences exposure and response to rituximab: pharmacokinetic-pharmacodynamic modeling using a syngeneic bioluminescent murine model expressing human CD20. Blood. 2009 Apr 16;113(16):3765-72. doi: 10.1182/blood-2008-08-175125. Epub 2008 Nov 24. PubMed PMID: 19029438.
  7. Aung T, Chapuy B, Vogel D, Wenzel D, Oppermann M, Lahmann M, et al. Exosomal evasion of humoral immunotherapy in aggressive B-cell lymphoma modulated by ATP-binding cassette transporter A3. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15336-41. doi: 10.1073/pnas.1102855108. Epub 2011 Aug 25. PubMed PMID: 21873242. (Free PMC Article available, 6 images available)
  8. Schmidt E, Hennig K, Mengede C, Zillikens D, Kromminga A. Immunogenicity of rituximab in patients with severe pemphigus. Clin Immunol. 2009 Sep;132(3):334-41. doi: 10.1016/j.clim.2009.05.007. Epub 2009 Jun 7. PubMed PMID: 19502112.
  9. McDonald V, Manns K, Mackie IJ, Machin SJ, Scully MA. Rituximab pharmacokinetics during the management of acute idiopathic thrombotic thrombocytopenic purpura. J Thromb Haemost. 2010 Jun;8(6):1201-8. doi: 10.1111/j.1538-7836.2010.03818.x. Epub 2010 Feb 19. PubMed PMID: 20175870.
  10. Kagan L, Turner MR, Balu-Iyer SV, Mager DE. Subcutaneous absorption of monoclonal antibodies: role of dose, site of injection, and injection volume on rituximab pharmacokinetics in rats. Pharm Res. 2012 Feb;29(2):490-9. doi: 10.1007/s11095-011-0578-3. Epub 2011 Sep 2. PubMed PMID: 21887597.
  11. Kagan L, Mager DE. Mechanisms of subcutaneous absorption of rituximab in rats. Drug Metab Dispos. 2013 Jan;41(1):248-55. doi: 10.1124/dmd.112.048496. Epub 2012 Nov 5. PubMed PMID: 23129212.
  12. Cragg MS, Bayne MB, Tutt AL, French RR, Beers S, Glennie MJ, et al. A new anti-idiotype antibody capable of binding rituximab on the surface of lymphoma cells. Blood. 2004 Oct 15;104(8):2540-2. Epub 2004 Jun 22. PubMed PMID: 15213098.
Stockage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light. 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G
PBS, pH 7.4
Agent Conservateur:
0.09% Sodium Azide
1% BSA
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Liquid purified IgG fraction from Tissue Culture Supernatant
This antibody specifically recognizes the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. This antibody does not recognise other CD20 antibodies.
It has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab (3).

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