discontinued

NB120-10345 PSTAIR motif anticorps

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Mouse anti Birds, Bovine, Human, Monkey, Mouse, Plant, Rat, Xenopus PSTAIR motif PSTAIR

NB120-10345

Description du Produit PSTAIR motif

Mouse anti Birds, Bovine, Human, Monkey, Mouse, Plant, Rat, Xenopus PSTAIR motif PSTAIR.
Presentation: Ascites
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Caractéristiques du Produit PSTAIR motif

Type de Produit Anticorps Primaires
Quantité 0.1 ml
Synonymes EGVPSTAIREISLLKE, PSTAIRE
Presentation Ascites
Reactivité d'Espèce Av, Bov, Hu, Mky, Ms, Pl, Rt, Xen
Applications E, IP, WB
Clonalité Monoclonal
Clone PSTAIR
Hôte Mouse
Isotype IgG1
Destination Not USA/Canada
Fiche Technique Télécharger la fiche technique
Fournisseur Novus Biologicals Inc.

Extrait de la Fiche Technique

Immunogène
Immunogène:
Synthetic peptide: EGVPSTAIREISLLKE conjugated to BSA.
Applications ELISA, immunoprecipitation, Western Blot, ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. It cannot precipitate p34cdc2 when it is complexed with cyclin B.WB: Use at a dilution of 1/4000 (determined by indirect blotting using COS-7 cell extract). The antibody recognises 31-34kD proteins (1-4) bands.Monoclonal Anti-PSTAIR may be used for the localization of cyclin-dependent kinases, containing the PSTAIR motif.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., ELISA, immunoprecipitation, Western Blot
Sujet The cell cycle is controlled in part by cyclin-dependent kinases (CDKs) which are themselves controlled by modifications such as phosphorylation of CDKs and formation of complex(es) with other proteins, including the cyclins. CDKs are key regulators of cell cycle progression. CDKs are closely related in size (35-40 kD) and sequence (>40% identity) and associate with and are activated by a cyclin which acts as a regulatory subunit. In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in the complex formation with cyclins. The availability of antibodies reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.
Références Générales General / background references:Morgan DO Principles of CDK regulation. Nature 374:131-4 (1995). PubMed PMID: 7877684
Mueller PR et al. Myt1: a membrane-associated inhibitory kinase that phosphorylates Cdc2 on both threonine-14 and tyrosine-15. Science 270:86-90 (1995). PubMed PMID: 7569953
Hunter T & Pines J Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age. Cell 79:573-82 (1994). PubMed PMID: 7954824
Draetta G Cdc2 activation: the interplay of cyclin binding and Thr161 phosphorylation. Trends Cell Biol 3:287-9 (1993). PubMed PMID: 14731844
Freeman RS & Donoghue DJ Protein kinases and protooncogenes: biochemical regulators of the eukaryotic cell cycle. Biochemistry 30:2293-302 (1991). PubMed PMID: 2001361
Stockage Store at -20C. Avoid freeze-thaw cycles.
Presentation
Purification:
Ascites
Buffer:
0.1% Sodium Azide
Pureté:
Ascites
Format:
Ascites
Specificité
Specificité:
Recognizes an epitope present in the PSTAIR sequence 1 of p34cdc2 (cdk1) and of other cyclin-dependent kinases containing the PSTAIR motif (cdk2 and cdk3)., PSTAIR (PSTAIR)
Espèces:
Avian, Bovine, Human, Mouse, Plant, Primate, Rat, Xenopus

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