BP7167 Protein Kinase C (PKC) gamma pThr514 anticorps

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Rabbit anti Human, Mouse, Rat Protein Kinase C (PKC) gamma pThr514


Description du Produit Protein Kinase C (PKC) gamma pThr514

Rabbit anti Human, Mouse, Rat Protein Kinase C (PKC) gamma pThr514.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Caractéristiques du Produit Protein Kinase C (PKC) gamma pThr514

Type de Produit Anticorps Primaires
Quantité 0.1 ml
Presentation Aff - Purified
Reactivité d'Espèce Hu, Ms, Rt
Applications WB
Clonalité Polyclonal
Hôte Rabbit
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCγ that contains threonine 514.
Applications The antibody has been used for Western blotting. For Western blotting applications, we recommend using the antibody at 0.5-1.0 µg/mL. At 0.50 µg/mL, the dilution provides 100 mL working solution, which at 10 mL/blot allows 10 blots to be performed. Positive controls used: Hela cells treated with PMA, a phorbol ester. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
Références Générales Wagner, L.M., et al. (2002) Effect of protein kinase Cγ on gap junction disassembly in lens epithelial cells and retinal cells in culture. Mol. Vis. 8:59-66.
Dutil, E.M., et al. (1998) Regulation of conventional protein kinase C isozymes by phosphoinositide-dependent kinase 1 (PDK-1). Curr. Biol. 8(25):1366-1375.
Toker, A. (1998) Signaling through protein kinase C. Front. Biosci. 3:D1134-D1147.
Machide, M., et al. (1998) Selective activation of phospholipase C γ1 and distinct protein kinase C subspecies in intracellular signaling by hepatocyte growth factor/scatter factor in primary cultured rat neocortical cells. J. Neurochem. 71(2):592-602.
Battaini, F., et al. (1995) Protein kinase C activity, translocation, and conventional isoforms in aging rat brain. Neurobiol. Aging 16(2):137-148.
Quest, A.F. and R.M. Bell (1994) The regulatory region of protein kinase Cγ. Studies of phorbol ester binding to individual and combined functional segments expressed as glutathione S transferase fusion proteins indicate a complex mechanism of regulation by phospholipids, phorbol esters, and divalent cations. J. Biol. Chem. 269(31):20000-20012.
Stockage Store at 4°C short term only. Aliquot and store at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Aff - Purified
PKCγ is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKCγ plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKCγ) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Reacts with human PKCγ. Mouse and rat (88% homologous) PKCγ have not been tested, but are expected to react. PKCα, ι, ζ (75%), βI, βII, ε and η (63%) may cross-react in cells expressing high levels of these proteins.

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