SM1691X Pk (V5) Epitope Tag (GKPIPNPLLGLDST) anticorps

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2 mg / 1 250,00 €
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Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-Pk1

SM1691X

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Description du Produit Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-Pk1.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Caractéristiques du Produit Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Type de Produit Anticorps Primaires
Quantité 2 mg
Presentation Purified
Applications C, E, IP, R, WB
Clonalité Monoclonal
Clone SV5-Pk1
Hôte Mouse
Isotype IgG2a
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur Acris Antibodies GmbH
Material Safety DataSheet (MSDS) MSDS for Monoclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Isotype Control AM03096PU-N
Add. information This product is manufactued under an exclusive license from the University of St. Andrews,UK.
Applications ELISA (1/1000-1/5000).
Western blot (1/1000-1/5000).
Immunoprecipitation.
Immunofluorescence.
Radioimmunoassays.
Immunohistochemistry on Frozen Sections.
Sujet The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Concentration 1.0 mg/ml
Références Générales
  1. Southern JA, Young DF, Heaney F, Baumgärtner WK, Randall RE. Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J Gen Virol. 1991 Jul;72 ( Pt 7):1551-7. PubMed PMID: 1713260.
  2. Hanke T, Szawlowski P, Randall RE. Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J Gen Virol. 1992 Mar;73 ( Pt 3):653-60. PubMed PMID: 1372038.
  3. Randall RE, Hanke T, Young D, Southern JA. Two-tag purification of recombinant proteins for the construction of solid matrix-antibody-antigen (SMAA) complexes as vaccines. Vaccine. 1993 Sep;11(12):1247-52. PubMed PMID: 7504859.
  4. Randall RE, Young D, Hanke T, Szawlowski P, Botting C. Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming. Vaccine. 1994 Mar;12(4):351-8. PubMed PMID: 8178558.
  5. Hanke T, Young DF, Doyle C, Jones I, Randall RE. Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding. J Virol Methods. 1995 May;53(1):149-56. PubMed PMID: 7543487.
  6. Jaffray, E. et al. (1995) Domain structure of IkBa and sites of interaction with NF-kB p65. Mol. Cell. Biol. 15: 2166-2172.
  7. Rodriguez MS, Michalopoulos I, Arenzana-Seisdedos F, Hay RT. Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol Cell Biol. 1995 May;15(5):2413-9. PubMed PMID: 7739525. (Free PMC Article available)
  8. Arenzana-Seisdedos, F. et al. (1995) Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol. Cell. Biol. 15: 2689-2696.
  9. Hirst, K. et al. (1994) The transcription factor, CDK, its cyclin and their regulator; directing the transcription response to a nutritional signal. EMBO J. 13: 5410-5420.
  10. Dunn C, O'Dowd A, Randall RE. Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J Immunol Methods. 1999 Apr 22;224(1-2):141-50. PubMed PMID: 10357214.
  11. Young DF, Chatziandreou N, He B, Goodbourn S, Lamb RA, Randall RE. Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells. J Virol. 2001 Apr;75(7):3363-70. PubMed PMID: 11238862. (Free PMC Article available, 6 images available)
  12. Sanchez Garcia J, Ciufo LF, Yang X, Kearsey SE, MacNeill SA. The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit. Nucleic Acids Res. 2004 Jun 1;32(10):3005-16. Print 2004. PubMed PMID: 15173383. (Free PMC Article available, 6 images available)
Stockage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Presentation
Purification:
Affinity Chromatography on Protein G
Buffer:
PBS
Agent Conservateur:
0.09% Sodium Azide
Pureté:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Format:
Liquid purified IgG fraction
Purified
Specificité
Specificité:
This antibody recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
This antibody has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).

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