discontinued

AP33097PU-N mCherry anticorps

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Goat anti mCherry

AP33097PU-N

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Description du Produit mCherry

Goat anti mCherry.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Paraffin Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Caractéristiques du Produit mCherry

Type de Produit Anticorps Primaires
Quantité 1.5 mg
Synonymes Cherry Fluorescent Protein, Red Fluorescent Protein
Presentation Aff - Purified
Applications C, ICC/IF, P, WB
Clonalité Polyclonal
Hôte Goat
Isotype IgG
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur Acris Antibodies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Immunogène:
Purified recombinant peptide produced in E. coli.
Applications Western blot: 1/500-1/2,000. 
Immunofluorescence: 1/50-1/500. 
Immunohistochemistry on Paraffin Sections: 1/50-1/500.
Immunohistochemistry on Frozen Sections: 1/50-1/500.
Sujet mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. GFP was the basis of the 2008 Nobel Prize in Chemistry, awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien, specifically "for the discovery and development of the green fluorescent protein, GFP". On expression from the GFP gene, GFP protein will fold correctly and fluoresce strongly, the development of fluoresence requires no cofactors except molecular oxygen. It can therefore be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell under aerobic conditions. As a result DNA encoding GFP can be fused to DNA encoding other proteins as a means to visualize the resulting fusion protein in live cells or animals. Engineered forms of GFP and relatives have been developed to monitor Calcium levels, protease activation and in a variety of other processes in real time. A whole range of GFP derivatives with different spectral properties have been developed, largely in the Tsien lab. The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome.
Concentration 3.0 mg/ml
Stockage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Presentation
Purification:
Epitope Affinity Chromatography
Buffer:
PBS
Agent Conservateur:
0.05% Sodium Azide
Stabilisateurs:
20% Glycerol
Format:
Liquid purified IgG fraction
Aff - Purified
Specificité
Specificité:
In 293HEK cells transfected with cds plasmid detects a band of approximately 30 kDa by Western blot.
This mCherry antibody Cat.-No AP33097PU is specific for mCherry and does not recognizes GFP (Green Fluorescent Protein). 
Reacts against mCherry, Red Fluorescent Protein from so-called disc corals of Discosoma species.

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