AP07900PU-N BLM (1319-1335) anticorps

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50 µg / 380,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Vue générale rapide

Rabbit anti Human BLM

Description du Produit BLM

Rabbit anti Human BLM.
Properties: (1319-1335)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections.

Caractéristiques du Produit BLM

Type de Produit Anticorps Primaires
Target Category
Quantité 50 µg
Synonymes Bloom syndrome protein, DNA helicase, RECQ2, RECQL3, RecQ protein-like 3, RecQ-like type 2
Presentation Purified
Reactivité d'Espèce Hu
Applications E, P
Clonalité Polyclonal
Hôte Rabbit
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur OriGene Technologies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Swiss Prot:
P54132
Immunogène:
Synthetic peptide from human BLM, aa 1319-1335
GeneID:
641
Caractéristiques 1319-1335
Applications ELISA: 1/1000.
Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Sujet The Bloom's syndrome (BS) gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DExH box-containing DNA and RNA helicases. The BLM protein has similarity to 2 other proteins that are members of the subfamily, namely the gene product encoded by RECQL2, also called the Werner syndrome gene (WRN), and the product of the yeast gene SGS1. These proteins may interact with topoisomerases, have 42 to 44% amino acid identity across the conserved helicase motifs, are of similar length and contain highly negatively charged N-terminal regions and highly positively charged C-terminal regions.
The BLM protein is located in the nucleus of normal human cells in the nuclear domain 10 (ND10) or promyelocytic leukemia nuclear (PML) bodies. These structures are punctate deposits of proteins disrupted upon viral infection and in certain human malignancies. BLM was found primarily in ND10 except during S phase, when it colocalized with the Werner syndrome gene product, WRN, in the nucleolus. The BLM protein is likely to be part of a DNA surveillance mechanism operating during S phase - BLM was found to be part of the BASC (BRCA1-associated genome surveillance) complex, which may serve as a sensor of abnormal DNA structures and/or as a regulator of the postreplication repair process.
Bloom syndrome cells show marked genomic instability; in particular, hyperrecombination between sister chromatids and homologous chromosomes - SCE (sister chromatid exchanges). In vitro BLM selectively binds Holliday junctions formed during genetic recombination and acts on recombination intermediates containing a Holliday junction to promote ATP-dependent branch migration. BLM may disrupt potentially recombinogenic molecules that arise at sites of stalled replication forks.
Concentration 1 mg/ml
Stockage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
Presentation
Purification:
Protein G Chromatography
Buffer:
Phosphate Buffered Saline PBS containing 0.09% Sodium Azide as preservative
Format:
Liquid purified IgG fraction
Purified
Reactivité d'Espèce
Reactivité d'Espèce (Testé):
Human
Specificité
Specificité:
Recognises Bloom's Syndrome Protein (BLM) at aa 1319-1335.
ID Gène 641

Accessory Products

Protéines et/ou Positive Controls

Protéines pour BLM (2 produits)

N° du Produit Espèces Presentation Pureté   Source  

BLM

BLM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

BLM

BLM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.
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