AP20103PU-N Alcohol Oxidase anticorps

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1 mg / 450,00 €
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Rabbit anti Candida Alcohol Oxidase

AP20103PU-N

Description du Produit Alcohol Oxidase

Rabbit anti Candida Alcohol Oxidase.
Presentation: Purified
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Caractéristiques du Produit Alcohol Oxidase

Type de Produit Anticorps Primaires
Quantité 1 mg
Synonymes AOD1, AOX, MOX, Methanol Oxidase
Presentation Purified
Reactivité d'Espèce Candida
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonalité Polyclonal
Hôte Rabbit
Isotype IgG
Destination Worldwide
Fiche Technique Télécharger la fiche technique
Fournisseur Acris Antibodies GmbH
Material Safety DataSheet (MSDS) MSDS for Polyclonal Antibodies (de)

Extrait de la Fiche Technique

Immunogène
Swiss Prot:
Q00922
Immunogène:
Alcohol oxidase isolated and purified from Candida boidinii.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Applications This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques, to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
Working Dilutions:
Non-precipitating antibody-binding techniques: 1/1,000-1/60,000.
Sujet Alcohol Oxidase (AOX) is a homooctameric flavoprotein consisting of eight identical subunits of ~74 kD, each containing a flavin adenine dinucleotide molecule (FAD) as a prosthetic group (van der Klei et al., 1991). The protein catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of certain yeasts including Pichia pastoris, Hansenula polymorpha, and Candida boidinii (van der Klei et al., 1991). AOX is normally localized in the matrix of single membrane-bound organelles called peroxisomes. During methanol growth, the peroxisomes also contain large amounts of dihydroxyacetone synthase, the first enzyme in the methanol assimilatory pathway, and catalase (CAT), which converts the hydrogen peroxide generated by oxidases such as AOX into water and oxygen (Veenhuis and Harder, 1991). As a result, peroxisomes, which are small and few in number in glucose-grown cells, are massively induced in methanol-grown cells (Veenhuis and Harder, 1991).
Concentration 2.0 mg/ml
Stockage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Shelf life: one year from despatch.
Presentation
Purification:
Solid Phase Affinity Chromatography
Buffer:
PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins
Format:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore by adding 0.5 ml of sterile distilled water
Specificité
Specificité:
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Espèces:
Candida boidinii.

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